A memory including reference cells is provided. The seat assembly includes a seat and a coupling tube laterally extended under the seat. In one mode the steam generator generates a flow of steam and fluid. The preferred route can be adapted to override an initial route. The inner coaxial tube andor one or more secondary cannulation in the wall of the outer coaxial tube terminate at proximal and as MDI adapters and at the distal tip as a single or multiple micrometric orifices. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting from bacterial infection.