This invention is directed to a class of miniribozymes, capable of hybridizing with a target RNA to be cleaved and exhibiting very high cleavage rates at low Mg2 concentration. The switch is turned on by a portion of the battery cover pressing the switch when the battery cover is assembled to the housing. The hook is disengagably engaged with the groove so as to prevent the shaft from being rotated when the release button is unintentionally touched. The tabbottle captwist cap opener can be formed integrally to a drinking vessel, on a bottom plate secured to a drinking vessel, as a stand-alone opener or as part of a novelty item such as a picture frame, coaster, or sports article such as a hockey puck or racing wheel, or a picture frame. Genomic DNA isolated according to the inventive subject matter is substantially free of polymerase inhibitors and of sufficient quality for quantitative PCR.