A spectral characterization method accurately characterizes an applied signal spectrum that has multiple, spectrally-separated signal peaks. The swim cap is generally open at the bottom and includes a forward edge, a rearward edge, oppositely disposed ear cover portions, and a center portion. The contaminant-containing stream is separated into an intermediate pressure carbon dioxide-enriched vapor stream and an intermediate pressure solvent and contaminant-enriched stream in the intermediate pressure separator. A first air chamber is on a first side of the first diaphragm opposite the hydraulic chamber and a second air chamber is disposed on a second side of the second diaphragm opposite the hydraulic chamber. An additional correction step can further ensure that the corrected positions correspond to the preset fixed distance of the localizers. Oligo nucleotide primers and kits used to amplify regions of a CFTR nucleic acid for high throughput, massively parallel sequencing and methods of determining an individual’s cystic fibrosis status are also disclosed.