A method of operating a vehicle including an engine is provided The engine may include at least one cylinder, a boosting device to boost intake air to the at least one cylinder, a fuel tank, a fuel vapor canister to store fuel vapors vented from the fuel tank, and an emission control device to treat exhaust gas from the engine. These novel nucleic acids are useful for constructing the claimed DNA vectors and host cells of the invention and for preparing the claimed recombinant proteins and antibodies that are useful in the claimed methods and medical uses. A recess cavity is formed in the substrate adjacent to the gate stack. Connected to the playback terminal 13 is an audio transducer 15, and a user control device 17. The lower leg is furnished with one or more projections, to make it possible to use handling equipment.