The present invention relates to a method and kit for detecting antibodies in clinical samples of animals infected with equine infectious anemia virus using the immunodiagnosis with the recombinant viral antigen p26. A method is described for providing a visual indication when an item, such as the cover, needs to be replaced. Creation of 3D laser-induced head image has three stages. A recess is defined in the upper wall. The device further includes a gate structure formed over the surface of the substrate between the active regions.