Disclosed is a method of preparing Tricholoma matsutake-infected young pine trees by coculturing aseptic seedlings of a pine tree with a T. Plants are characteristically strong and resistant to breaking, and produce dark green foliage. A bearing is attached to an inner periphery of the brake section. The resilient elongate orthopedic device may be restrained to a reduced profile that permits minimally invasive implantation, but assume an enlarged profile when positioned at an implantation site. By the composition of above structure, a part of a quick release handle connected to a seat tube and a middle tube of a bicycle seat may be locked, and a linked locking mechanism of an external lock latch may be provided simultaneously, to thereby achieve the purpose of preventing theft from bicycle seat, front and rear wheel or other objects within a bicycle.