A medical syringe contains neoprene O-rings and is constructed such that its nozzle and handle can be sterilized without damaging the O-rings. The antagonists of this invention typically are selected from among several classes but preferably are soluble TNF receptors. Pairing of modulated colors using relative luminance helps to minimize flicker effects. As such, the latency from the time the last sample is received and the availability of the coefficients is decreased. Steps include growing a lower mirror on a substrate; growing an active region on the lower mirror; growing an upper mirror on the active region; depositing a grating layer on the upper mirror; and etching a grating into the grating layer. It also discloses an efficient formula to make a mixed SAM that can greatly enhance the immobilization ability of the metal surface in SPR based techniques, which is good for the immobilization of relevant antibodies and antigens used for the detection of respective thyroid hormones and related antibodies in a serum sample.