An animal training device having a housing, a sound-producing member, an actuator, and a whistle. Some of the polymer embodiments are heparin binding polymers. Lipids or lipids and enzymes are produced by said microorganisms in the single cell oil production process andor in a process connected into it. The memory circuit has two control inputs, one to a first switch between the input terminal and an input buffer and the other to a second switch between the serial data path and the input buffer. The casing includes a set of electrical connections including at least one radio-frequency differential line between the chamber and the casing exterior. By employing low acid catalyst levels compared to amounts conventionally used, rapid formation of acetal or ketal is facilitated while the formation of oxocarboxylate esters is minimized.