The present invention provides nucleic acid compositions encoding a novel colorless GFP-like protein, acGFP, from Aequorea coerulscens and fluorescent and non-fluorescent mutants and derivatives thereof, as well as peptides and proteins encoded by these nucleic acid compositions. A fill valve communicates with pressurized fluid in the inlet port. A variety of different audio processing modules can be used to provide various pieces of functionality when processing audio data.