This invention provides a patch with less skin stimulation, excellent in long time store stability and heat stability and having favorable tack during use. When the tip up is set for catching fish, the spool can rotate about its axis a limited distance before the spring jerks the shaft upwardly to set the hook. For such a purpose, the inner inflatable bodies are inflated, in particular, by a higher pressure than the outer inflatable body. To amplify the signal, beads coated with antibodies are introduced and the beads would stick to the pathogen causing a greater change in impedance between the two adjacent electrodes. The terminal base soldered to the substrate is prevented from being peeled off by eliminating twisting force applied to the spring-contact member. The two LEDs are placed on a conductive substrate ; therefore, the bonding pads of the two LEDs are on the back of the conductive substrate and the light emitting surfaces of the two LEDs, thus overcoming such problems of low light emitting efficiency and high thermal resistance of the traditional lateral structure.