A non-aqueous pigment ink is provided, wherein the non-aqueous pigment ink comprises an alkyl acrylate copolymer containing an alkyl acrylate unit having an alkyl group of 12 or more carbon atoms and a acrylate unit having a pyridyl group, a pigment, and a non-aqueous solvent. The microfluidic card has a plurality of channels and ports on a top surface of the card in a desired configuration, the ports in fluid communication with the channels. In one embodiment, an output bypass path is used for directly connecting the output of the LE to the input of an IO multiplexer of an IO block. Various open and closed cooling systems are described. The pump and valve assemblies may be controlled either mechanically or electrically. Related methods and kits are also provided.