A composition comprising a substantially purified compound of the formula:in combination with at least one additional therapeutic agent, and methods of preventing or treating cancer and a condition treatable by the inhibition of vacuolar-type -ATPase. The hydrofluorocarbon compound forms a hydrofluorocarbon polymer layer on sidewalls of the at least one opening and helps to preserve the profile of the at least one opening. The dispenser is moved to provide agitation to the dispenser for mixing the bonding adhesive into a homogeneous mixture of particles and the liquid carrier. The application module has computer readable instructions.