Methods for the rapid detection of the presence or absence of a SNP in a target nucleic acid in a sample are described. All of spread transmission data are additively combined with each other in an additive composite section 11 to obtain an amplitude data S2 and a transmission mean value information S8 at its optional time mean. In other aspects, the methods encompass administering to the subject an amount of a glutamate inhibitor effective to treat the demyelinating condition. Therefore, a completing process takes place before a VOBU boundary, and a completed PCK will not be generated immediately after the VOBU boundary. The server also maintains information on the authorized uses made by each of the jukeboxes and determines the amount of royalties due.