A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. In this composition and method, an active material having the formula given below is applied to the patient at the site of said condition:wherein R1 is selected from \u2014OH and C1-C3 alkyl OH; and R2, R3, R4, R5 and R6 are independently selected from \u2014H, \u2014OH, C1-C6 alkyl, and C3-C6 cycloalkyl. In a bottom-gate transistor including an oxide semiconductor layer, an oxide semiconductor layer functioning as a current path of the transistor is sandwiched between oxide semiconductor layers having lower carrier densities than the oxide semiconductor layer. An output signal may be generated that is representative of the estimated and actual rates and the estimated and actual volumes of data for the at least one subprocess. The secondary generator stores the value and forwards the value to the originating entity.