In certain aspects, the present invention provides methods for increasing the replication capacity of influenza viruses in hens’ eggs andor cell culture, recombinant andor reassortant influenza viruses with increased replication capacity, and immunogenic and vaccine compositions comprising such recombinant andor reassortant influenza viruses. The device includes a member, such as an inflatable balloon or a self-expanding basket, for obstructing a first passage. The multiple interfaces within the IOC manage packets and messages according multiple different protocols.