The invention provides an assay method for detection andor quantification of a plurality of nucleic acid or protein targets in a sample. Examples of certificate related actions include certificate enrollment, renewal, and revocation. When registers are no longer allocated according to the ideal allocation map and the registers are currently allocated according to the external allocation map, the registers are deallocated in the external map and the number of freed registers is reported to the master. The method further includes receiving, at the proxy server, a data request.