The invention is directed to modified T cells, methods of making and using isolated, modified T cells, and methods of using these isolated, modified T cells to address diseases and disorders. The connection structure comprises a supporting body of the pressure-sensitive component, which defines a respective passage, and a compressible element that is designed to be in contact with the fluid and is configured for compensating possible variations of volume thereof. The extraction filter specifically binds to a molecule of interest. The present invention in various implementations provides an intelligent prediction scheme for populating and depopulating multiple applications at the system level across a diversity of applications.