A tissue collection apparatus for initiating bone formation ex vivo. Specifically, the gate oxide films comprise a first gate oxide film 23 and a second gate oxide film 25 formed on the first gate oxide film 23; the plurality of silicon particles 24 are located between the first gate oxide film 23 and the second gate oxide film 25, and the first gate oxide film 23 is formed in an extremely thin thickness. Several feeder tubes extend outwardly from the lower end of the coupling tube and together with the lower end of the coupling tube define a central chamber of the discharge unit. A sheet laminating slot is defined between the diffuse and the press roll.