An apparatus and method is provided for managing and controlling connections to conference bridges for proposed conference call participants to access a scheduled conference call. The present disclosure also provides a method using the pharmaceutical composition for detecting human melanoma cells and a kit performing the method. The method also includes recording a synthesized speech output generated by a text-to-speech engine, the synthesized speech output being an audible rendering of the pre-selected text, and parsing the synthesized speech output into individual words and periods of silence. The second conductive type well includes a well lower surface, which has a first part and a second part, wherein the first part is directly above the buried layer and electrically coupled to the buried layer; and the second part is not located above the buried layer and forms a PN junction with the substrate.