The present invention is directed to DNA encoding human endothelial cell growth factors, and to plasmids comprising said DNA. A substrate having an uneven surface topology is provided. The first pass further includes grouping the set of pixels into blocks of pixels and writing each of the blocks of pixels to a memory location. After the theoretical mass flow rate is calculated, reference is made to a theoretical mass flow rate-discharge coefficient correspondence table recorded in a memory corresponding to the classification of the gas to select a desired discharge coefficient.