Systems for removing a tire from a rim are shown and described. An initial build of a computer system, modifications to an existing computer system configuration, extended services, and enhancements are incorporated. Each test object has one or more associated test methods and each test method can describe one or more state transitions. The cyclonic airflow chamber causes the suction airstream to travel in a cyclonic path such that the entrained contaminants are separated therefrom and deposited into a dirt container that defines the chamber. The invention further relates to diagnostic and therapeutic methods useful for diagnosing, treating, preventing andor prognosing disorders related to these novel polypeptides.