The present disclosure relates to novel methods of discriminating andor detecting mis-matched polynucleotide populations in a sample by determining the ratios of mismatched polynucleotide species after specific enzymatic digestion treatment. or higher; wherein the mass ratio of the polyolefin and the saponified ethylene-vinyl acetate copolymer is 60:40 to 99. If the base station determines a failure to decode any uplink signals, instead of sending acknowledgement signals over the indicator channel, the base station may, instead, generate uplink grants for retransmission of the uplink signals. Cameras detect objects and generate image signal.