Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. The method provides contacting a permeabilized cell with labeled C\u03b8 complex. The vectors may be indicative of an output of the three phase system. A determination on the sufficiency of funds in a pre-paid account is made.