A method of treating a substrate for immobilizing a biomolecule and substrates produced by the method are disclosed. An effervescent formulation comprising oxytocin wherein dissolution in water of the multilayer effervescent microspheres leads, after almost immediate effervescence, to a solution or a homogeneous dispersion of the oxytocin. The light produces changes in at least one optical element of the projection exposure apparatus, by which the optical properties of the projection exposure apparatus are influenced. In case the porous carrier pieces are used, the reactivity of the inner surfaces of the porous carrier pores should be maintained during array or immobilization process of the reactive probe-loaded carrier. 5 inch width after the multi-layer assembly has been stored for 46 days at 120\xb0 F.