Methods of determining whether a sample includes one or more analytes, particularly proteinaceous analytes, of interest are provided. In one example, a critical temperature is determined for the lead that is representative, e. The disc control informations are recorded for at least one write strategy for a same writing speed and recording layer. In still another embodiment, shared media element are provided for the PII and IMS. The lower-level interconnects and the upper-level interconnects are connected with each other through vias.