A holder for PCR sample collection and preparation and method of using which has a buffer container housing removably connected to a plunger housing. The glass pane includes two bus bars which are located parallel to a base edge of the glass pane and arranged essentially in a line in their axial direction, spaced apart a short distance from one another. Various embodiments of the present invention may also further provide a system and method for performing a minimally invasive laminectomy procedure via the midline approach described above that may thus reduce the trauma experienced by tissues surrounding the spine or other bony structure.