A method for detecting DNA damage in a tissue sample involves contacting an immobilized biological sample with a labeled ligand which binds to human 53Bp1, and examining the immobilized sample for the presence of a label generated-detectable signal concentrated in foci in said sample. According to one embodiment of the present invention, the lining includes a first covering layer facing the engine. The first sash is connected to a first axle, wherein the first axle is adjacent to the first end of the rail. The format command is enabled in dependence upon the comparison between the format command key and the recorded key. If the remote is outside the transmission field and is therefore unresponsive to the interrogating signal, the microprocessor initiates various security measures such as locking doors, disabling the engine and arming the security system.