Quantitative and qualitative analysis of a nucleic acid analyte in a sample suspected to contain the nucleic acid analyte if achieved by first preparing a reaction mixture containing the sample and a known amount of an internal quantitation standard. The supervisory device establishes the upstream slot structure and allocates upstream bandwidth by sending messages over its downstream channel. The first pressure is larger than the second pressure, and the adjusted compressed air is not output to the second pneumatic tool at the first pressure. A change of impedance of material of the object between the conductive ends can be sensed to provide information on the depth of penetration.