The invention relates to tubing handling apparatus for injecting tubing into and recovering the tubing from a wellbore. Starting with an aluminium and titanium alloy bar having a simple andor axisymmetric shape, at least two mutually interlocking blanks are produced in the bar by waterjet cutting, and then each one of said blanks thus obtained is machined separately in order to obtain the blades having a final profile. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein. The stem is positioned to frictionally engage the flexible fingers as the stem is rotated. The apparatus includes a water supply system in fluid communication with the open volume via nozzles for flowing water through and back-filling the volume in a manner that does not produce bubbles within the volume. During a water discharge mode, the hot water in the hot reservoir is removed from the storage tank and flows into a hot water discharge.