A refrigerant system, which utilizes CO2 as a refrigerant, includes a main closed-loop refrigerant circuit and a booster closed-loop refrigerant circuit. The cutting blade is particularly suited for cutting sheet material such as foil wrap from a roll. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-ethylglycine and related translation systems. By this arrangement, the heat developed along the length of the constant wattage heater as it passes through the thermal insulation of the substrate can be dissipated safely through the body of the conductive conduit.